Welcome to Dutta Lab
Protein Structure determination using cryo-electron microscopy
My group is focusing on the structural studies of polyketide synthase, pore forming toxin bacterial secretion system, bacterial ion channels and GPCRs using cryo-electron microscopy. Single particle cryo-electron microscopy (cryo-EM) techniques are developing rapidly for the structural characterization of complicated biological complexes. These methods generate snapshots of macromolecules in solution and under highly controlled biochemical conditions, a crucial condition for probing physiological structures of distinct functional states. Recent high impact publications suggest that cryo-EM has enough potential to resolve the high resolution structure of biological samples and is an essential structural biology instrument, which allows us to determine the three-dimensional (3D) structures of large biological macromolecules, macromolecular complexes, and cell components We use different biochemical, biophysical techniques including negative staining electron microscopy and cryo-EM techniques to characterize the conformational dynamics of very small (100 kDa) protein complexes to very large biological macromolecules (2.6 MDa).
Lab News :
1. Somnath Dutta received Ramanujan Fellowship
2. Early Career research Award of DST.
3. DBT Advanced Centre for Cryo-electron microscopy (Cryo-EM)
1. Somnath Dutta received Ramanujan Fellowship
2. Early Career research Award of DST.
3. DBT Advanced Centre for Cryo-electron microscopy (Cryo-EM)