Welcome to Dutta Lab
Protein Structure determination using cryo-electron microscopy
My group is focusing on the structural studies of polyketide synthase, pore forming toxin bacterial secretion system, bacterial ion channels and GPCRs using cryo-electron microscopy. Single particle cryo-electron microscopy (cryo-EM) techniques are developing rapidly for the structural characterization of complicated biological complexes. These methods generate snapshots of macromolecules in solution and under highly controlled biochemical conditions, a crucial condition for probing physiological structures of distinct functional states. Recent high impact publications suggest that cryo-EM has enough potential to resolve the high resolution structure of biological samples and is an essential structural biology instrument, which allows us to determine the three-dimensional (3D) structures of large biological macromolecules, macromolecular complexes, and cell components We use different biochemical, biophysical techniques including negative staining electron microscopy and cryo-EM techniques to characterize the conformational dynamics of very small (100 kDa) protein complexes to very large biological macromolecules (2.6 MDa).
Lab News :
1. Somnath Dutta received Ramanujan Fellowship
2. Early Career research Award of DST.
3. DBT Advanced Centre for Cryo-electron microscopy (Cryo-EM)
4. Haaris Ahsan Safdari and Ishika Pramanick, PhD student of our lab won the second and consolation prize respectively in the International Conference on Microscope and XXXIX Annual Meeting of Electron Microscope Society of India, Bhubaneshwar, India (2018)
5. The proposal for Foldscope by Haaris Ahsan Safdari gets approved under Category-C by the Department of Biotechnology, Ministry of Science and Technology, Govt. of India (2018)
1. Somnath Dutta received Ramanujan Fellowship
2. Early Career research Award of DST.
3. DBT Advanced Centre for Cryo-electron microscopy (Cryo-EM)
4. Haaris Ahsan Safdari and Ishika Pramanick, PhD student of our lab won the second and consolation prize respectively in the International Conference on Microscope and XXXIX Annual Meeting of Electron Microscope Society of India, Bhubaneshwar, India (2018)
5. The proposal for Foldscope by Haaris Ahsan Safdari gets approved under Category-C by the Department of Biotechnology, Ministry of Science and Technology, Govt. of India (2018)
